Police Scotland is proposing to close the control centre at the former Fife headquarters in Detroit Road, Glenrothes.
It plans to create a national command base in Inverness, and three area control centres, with Fife coming under the east area based at Bilston Glen in Midlothian.
There is also a plan to create ‘national virtual service centres’ at Motherwell, Govan in Glasgow and Bilston Glen.
The force says it has carried out a “comprehensive review” which identified that it could significantly improve its service by having fewer, but larger sites.
At present, Police Scotland operates 10 control rooms and nine service centres across 11 sites.
If the proposals get the go-ahead, this would be reduced to five sites over a two-year period.
Chief Superintendent Val Thomson said: “The current structure across the 11 sites is based on the arrangements which were in place under the previous eight force structure in Scotland.
“Since April 1 2013, we have operated to a high standard in terms of response to emergencies, dealing with non-emergency calls through 101 and managing incidents.
“Moving to fewer, larger centres will enhance our capability to respond to day-to-day incidents, as well as complex and large scale emergencies, with increased availability of staff, better technology and easier and faster ways to share information.”
Staff affected by the closure of the centre in Glenrothes will have options including relocation to the remaining centres, early retirement, voluntary redundancy or redeployment to other roles with Police Scotland.
The force says there will be no compulsory redundancies.
However, UNISON says the proposals will put up to 300 jobs at risk.
Gerry Crawley, Regional UNISON officer, said: “These closures are not about making our communities safety. They are budget driven cuts. The Scottish Government is cutting £139 million from police budgets between now and 2017 and £1 billion over 12 years.
“Closing these control centres puts 300 jobs at risk. We are concerned that 999 and 101 services will be less responsive and local communities having lost police stations across the country will see even more local cuts.”