It is currently being treated as unexplained.
Officers were called to Boblingen Way in Glenrothes at 1:40am after reports a man was injured in the street.
The area was cordoned off and emergency vehicles were also seen in nearby Forres Drive.
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Police confirmed the man was pronounced dead at the scene.
A spokesman for Police Scotland said: “Officers were called to Boblingen Way, Glenrothes, around 1:40am on Saturday to reports of a man was injured in the street.
“He was pronounced dead at the scene.”The spokesman added: “The death is being treated as unexplained, and inquiries are continuing.”