The business is making the short move into the former Buzz Bingo premises in Flemington Road, Glenrothes.
The doors to the new, bigger store will open on Tuesday, August 2.
B&M will close their current store in the same road on July 9.
The company got permission from Fife Council to make the move earlier this year.
A spokesman said: “The store will be much bigger than the previous with over 26,064sq. ft. of sales space, offering an even bigger selection of great bargains from grocery, toiletries and health & beauty to toys, homeware and DIY.
“It will also boast its own gardening centre selling hundreds of plant varieties and gardening essentials.
The spokesman added: “We wanted a bigger and better store for our customers, giving them even more big branded products.”
“We’re really excited to get the doors open.”