The church will also receives a £7500 Wolfson Fabric Repair Grant from the Wolfson Foundation.
Kirkcaldy Old Kirk is the oldest building in continuous use in town, and today is a non-denominational Christian building.
Rosemary Potter, who chairs Kirkcaldy Old Kirk Trust, said: "We are delighted to have this welcome support for our tower restoration project from the National Churches Trust, and the further assistance from the Wolfson Foundation.”She added: “This will help to secure the future of the ancient tower as a well-loved landmark in our town centre and inspire local people and visitors to connect with their heritage.
“We have recently seen the first services back in the church since Christmas and it is fitting that this tangible symbol of hope that has watched over the town for more than eight centuries will shortly be brought to renewed life after such a difficult year.
"We are grateful to the National Churches Trust and the Wolfson Foundation for investing in Kirkcaldy's future in this way and hope visitors to this church where Adam Smith was baptised in 1723 will enjoy climbing the 100 steps for the special panoramic view out to sea."
The church has a rich history.
It was founded by monks from Iona in the 7th century, it was re-consecrated by the Catholic Bishop de Bernham in 1244, and after the Reformation it became Kirkcaldy Parish church, with Revd George Gillespie playing a key part in securing Presbyterianism.
The 15th century, seven-metre square tower is the oldest part of the church, measuring 28 feet by 24 feet, the lower walls being five feet thick.
The tower was heightened at one time to increase the range of the bell which was first cast in 1553 and recast several times with it still being rung today.
The parapet walkway gives fine views over the town and the River Forth, with a unique historical perspective on the growth of the town.