We look back at some of the more recent clashes between the two sides.
. Stark's Park - Kirkcaldy - Fife - Raith v Ayr - GREIG SPENCE celebrates scoring - credit- Fife Photo Agency
Raith take on Ayr in 2018 (Pic: Fife Photo Agency) Photo: walter neilson
. Ayr United 0 Raith Rovers 2
Ross Callachan celebrates as Rovers open the 2016-17 Championship season with a 2-0 win at Somerset Park. Ross Matthews added the second. Photo: David Sargent
. Raith Rovers 1 Ayr United 1
Joel Coustrain scores for Raith in a 1-1 draw at home in November 2016. Photo: Fife Photo Agency
1. Ayr United 0 Raith Rovers 2
Ross Callachan celebrates as Rovers open the 2016-17 Championship season with a 2-0 win at Somerset Park. Ross Matthews added the second. Photo: David Sargent
2. Raith Rovers 1 Ayr United 1
Joel Coustrain scores for Raith in a 1-1 draw at home in November 2016. Photo: Fife Photo Agency
3. Ayr United 1 Raith Rovers 0
The notorious match where midfielder Ryan Stevenson had to go in goal occurred in February 2017. He is pictured with the scorer of the game's only goal, Ayr's Farid El Alagui. Photo: SNS Group - Ross MacDonald
4. Raith Rovers 2 Ayr United 1
Jonny Court scores his first goal for Raith in a 2-1 win at Stark's Park in May 2017. Declan McManus scored Rovers' second. Photo: Fife Photo Agency