The road will be closed from the junction of A914 Clushford Toll to the junction of C16 Cupar Muir road, Monday to Friday, from 7:30am until 5:00pm.
There will be access for emergency services and pedestrians but access for residents and businesses will be at the discretion of the contractor.
Bus services will be affected during the £87,000 resurfacing project.
Mark Dewar, service manager for roads and lighting contracts said: “Our road network is vital to support economic development, inward investment, tourism, leisure and travel to work. Almost everyone in Fife uses our roads on a daily basis, which is why this type of maintenance work is so essential.”
He added: “We apologise for any inconvenience and delay caused, and we’ll try to keep any disturbance to a minimum.”